《植物生理学报》 2017, 53(11): 2031-2036

两份大麦纯合突变体氮代谢相关基因于不同生长发育时期的表达

徐红卫^*, 郭桂梅^*, 黄赛华, 李颖波, 刘成洪, 陈志伟, 何婷, 高润红, 周龙华, 方春燕, 陆瑞菊^**, 黄剑华^**

上海市农业科学院生物技术研究所, 上海市农业遗传育种重点实验室, 上海201106

通信作者：徐红卫；E-mail: sw1@saas.sh.cn; luruiju62@163.com

摘　要：

以大麦(Hordeum vulgare)原始品种‘花30’作为对照, 经诱变结合小孢子氮胁迫培养获得的2份氮素利用率差异显著的纯合突变体A1-97和A1-57为供试材料, 利用盆钵种植方法, 研究其在低氮条件下不同时期氮代谢关键基因的表达。结果表明: 低氮水平下, 原始品种和氮素利用率较高的突变体A1-97中HvAS1基因的相对表达量在各时期均显著增加, 而氮素利用率较低的突变体A1-57中均显著降低; 相比正常供氮, 氮素利用率不同的突变体与原始品种中HvGS1_1基因的相对表达量在低氮水平下都显著增加。原始品种和突变体中基因相对表达量和不同时期的相关性分析结果表明, HvAS1基因的表达模式在苗期、灌浆期和成熟期3个时期之间均具有显著相关性。因此, 在低氮条件下, HvAS1和HvGS1_1基因可能与突变体材料的氮素利用率差异有关, 其中HvAS1在苗期即可进行分析。

关键词：大麦; 突变体; 氮代谢相关基因表达

收稿：2017-08-04   修定：2017-11-01

资助：上海市种业发展项目[沪农科种字(2016)第1-1号]、上海市市级农口系统青年人才成长计划[沪农青字(2017)第1-27号]和国家大麦青稞产业体系(CARS-05-01A-02)。

Expression of nitrogen metabolism-related genes in two barley homozygous mutants at different growing stages

XU Hong-Wei^*, GUO Gui-Mei^*, HUANG Sai-Hua, LI Ying-Bo, LIU Cheng-Hong, CHEN Zhi-Wei, HE Ting, GAO Run-Hong, ZHOU Long-Hua, FANG Chun-Yan, LU Rui-Ju^**, HUANG Jian-Hua^**

Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China

Corresponding author: XU Hong-Wei; E-mail: sw1@saas.sh.cn; luruiju62@163.com

Abstract:

In this study, we compared the barley (Hordeum vulgare) original cultivar (‘Hua 30’) with the mutants (A1-97 and A1-57) with high and low nitrogen utilization efficiencies (NUE) based on the microspore mutation. The expression patterns of key genes involved in nitrogen metabolism under two nitrogen levels at seedling, filling and mature stages were studied using the basin planting methods. The results showed that under the low nitrogen level, the expression of HvAS1 significantly increased in original cultivar and high NUE genotype (A1-97) at different stages, but the expression level in the low NUE genotype (A1-57) significantly reduced. The gene expression levels of HvGS1_1 were all significantly enhanced in the mutants and original cultivar under low-nitrogen condition. By analyzing the correlation between the different gene expression levels and different stages, the expression patterns of HvAS1 were significantly correlated among seedling, filling and mature stages. Therefore, HvAS1 and HvGS1_1 might be related with the difference in NUE of mutants under the low-nitrogen condition. Moreover, the HvAS1 can be preliminarily analyzed at the seedling stage.

Key words: barley; mutant; expression of nitrogen metabolism-related genes